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| 产品名称： | cTBQ4 |
|---|---|
| 商品货号： | TS133573 |
| Designations： | cTBQ4 |
| Species： | Homo sapiens, human |
| Applications： | Codominant segregation of the MspI RFLP was observed in 45 three-generation families. RFLPs were observed after competitive hybridization with total human DNA. |
| Vector： | Construct size (kb): 48.79999923706055 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D10S27 Alleles: A1, A2 |
| Insert Size (kb)： | 40.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--11.0,8.4,6.0,4.2,3.6,2.4,2.0; HindIII--18.0,5.0,4.0,3.4,2.8,2.1,1.3; BglII--15.0,6.8,5.2,4.2,2.9,1.7,1.3,1.1; SalI--39.0, 2.5; EcoRI--10.0,5.5,3.2,2.6,2.45,1.9,1.75. Codominant segregation of the MspI RFLP was observed in 45 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs were observed after competitive hybridization with total human DNA. Enzyme(s) not detecting polymorphism: TaqI, RsaI, BglII, PstI and PvuII. |
| References： | White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325 Raymond L White, personal communication |