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微信小章| 产品名称： | Shox2 |
|---|---|
| 商品货号： | TS133574 |
| Organism： | Mus musculus, mouse |
| Tissue： | sinoatrial node |
| Cell Type： | embryonic stem cell-derived nodal cardiac myocytes |
| Product Format： | frozen 1.0 mL |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryonic |
| Gender： | male |
| Strain： | 129S1/SVmJ |
| Applications： | Can be used for the design of biological pacemakers, studying the biology and electrophysiology of sinoatrial nodal cells, and in animal models of cardiac pacemaker diseases such as AV block or sinus node dysfunction. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Shox2 cell originated from the genetic selection of mouse ES cells using a 3.8 kb fragment of the Shox2 promoter. |
| Comments： | Shox2 cells originated from the genetic selection of mouse ES cells using a 3.8 kb fragment of the Shox2 promoter. They exhibit a pacemaker-like molecular phenotype and gene expression profile including pacemaker-specific transcription factors (Tbx3xa0 Tbx5 BMP4), structural proteins (alpha-cardiac actin; alpha-skeletal actin; desmin), connexins (Cx45; Cx30.2), ion channel subunits (Cav1.2; Cav1.3; Cav3.1), a sodium-calcium exchanger (NCX1) and a hyperpolarization activated cyclic nucleoside gated channel (HCN2).xa0 ( Hashem SI, et al. Genetic isolation of stem cell-derived pacemaker-nodal cardiac myocytes. Mol. Cell. Biochem. 383:161-171, 2013. PubMed: 23877224) |
| Complete Growth Medium： | The base medium for this cell line is Mouse ES Cell Basal Medium, Catalog No. SCRR-2011. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
xa0xa0 Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
xa0 Subcultivation Ratio: 1:3 to 1:6 is recommended. Medium Renewal: 2 to 3 times a week |
| Cryopreservation： | Freeze Medium: fetal bovine serum, 95%; DMSO, 5% Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C; Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Volume： | 1.0 mL |
| COI： | Mouse |
| Name of Depositor： | William Claycomb, Louisana State University |
| Year of Origin： | January 2012 |
| References： | Hashem SI, et al. Genetic isolation of stem cell-derived pacemaker-nodal cardiac myocytes. Mol. Cell. Biochem. 383:161-171, 2013. PubMed: 23877224 |