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| 产品名称： | pHTalpha2 UTR-01255, WI-7091 |
|---|---|
| 商品货号： | TS133614 |
| Designations： | pHTalpha2 UTR-01255, WI-7091 |
| GenBank Number： | X51420 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.799999952316284 Tissue: melanoma MeWo cell line Gene product: tyrosinase-related protein 1(homolog of b-locus protein, melanoma antigen gp 75) CAS2 Alleles: A2, A2, A1, A1, A2, A1 |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.8; BglII--5.8; AvaI--5.8; HincII--3.4, 2.4; PstI/PvuII--1.6, 1.5, 1.0, 0.8, 0.4. This clone, which was not derived from the same cell line as the published sequence (S7), does not have the HindIII site at nucleotide 748. This is probably due to polymorphism. It does have a BamHI site at 929. Either an AvaI/NdeI or an SmaI/NdeI digest gives a 1.7 kb fragment containing the protein-coding region except for a putative signal peptide. The vector lacks the SV40 promoter. This is a full length cDNA clone; coding sequences extend from nucleotide 130 through 1710. |
| References： | Cohen T, et al. Nucleotide sequence of the cDNA encoding human tyrosinase-related protein. Nucleic Acids Res. 18: 2807-2808, 1990. PubMed: 2111010 Shigeki Shibahara, personal communication |