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| 产品名称： | pBR328 |
|---|---|
| 商品货号： | TS133714 |
| Designations： | pBR328 |
| Depositors： | EM Lederberg |
| Biosafety Level： | 1 |
| Host： | Distribution host: E. coli RR1 |
| Vector Information： | Size (kb): 4.9000000953674320 Vector: pBR328 (plasmid) Promoters: Promoter none Construction: pBR327 ori, pBR325 cml Marker(s):ampR,cmlR,tetR Construct size (kb): 4.900000095367432 Features: marker(s): ampR, cmlR, tetR promoter: none replicon: pMB1 terminator: none |
| Comments： | A general purpose plasmid vector. Contains a 482 bp inverted duplication. Constructed from the PstI/BamHI fragment of pBR327 containing the replication origin and the PstI/BamHI fragment of pBR325 containing the cml gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Soberon X, et al. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9: 287-305, 1980. PubMed: 6248430 Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid. |