产品名称: | BL29-148 |
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商品货号: | TS133761 |
Designations: | BL29-148 |
GenBank Number: | T25841 T25842 |
Species: | Homo sapiens, human |
Depositors: | R Sanders |
Vector: | Construct size (kb): 3.650000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
Insert: | DNA: cDNA Insert lengths(kb): 0.75 Tissue: BL29 cell line Burkitts lymphoma Disease: Burkitts lymphoma Gene product: DNA Segment, numerous copies, expressed |
Insert Size (kb): | 0.75 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--3.8; EcoRI--2.9, 0.75; HindIII-- 3.8; PstI--3.8; SacI--3.8. |