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微信小章| 产品名称： | Vaccinia virus ts mutant |
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| 商品货号： | TS133773 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts47 (Dales isolate 6828) |
| Applications： | The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12. |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2 CDC BMBL recommends vaccination. ref
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12. |
| Effect on Host： | Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
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| Recommended Host： | Recommended Host: BSC-40 (ATCC® CRL-2761 |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent not 100% confluent for inoculation. |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | S Dales, RC Condit, S Dales |
| Special Collection： | ATCC |
| Source： | derived from existing strain |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4. or colder Non-permissive incubation temperature is 39.5C. This mutant was assigned a Dales EM category of P. It is assigned the Condit map location U12. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane. or colder |