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| 产品名称： | pHFTA plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS133796 |
| Designations： | pHFTA plasmid in Escherichia coli |
| GenBank Number： | L10413 |
| Species： | Homo sapiens, human |
| Depositors： | DA Andres |
| Vector： | Total size of construct (kb): 4.6 Vector: pBluescript SK+ Vector size (kb): 2.9 Markers: ampR Vector ends: EcoRI |
| Insert： | Insert size (kb): 1.67 Gene: farnesyltransferase, CAAX box, alpha, FNTA DNA: cDNA Insert ends: EcoRI Source: human Genbank accession: L10413 |
| Insert Size (kb)： | 1.670 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.67; EcoRV--3.9, 0.7; SmaI--4.3, 0.3; HindIII--4.6; PstI--3.1, 0.55, 0.35 (doublet), 0.25.
Transcription from the T3 promoter gives the sense strand. |
| References： | Andres DA, et al. cDNA cloning of the two subunits of human CAAX farnesyltransferase and chromosomal mapping of FNTA and FNTB loci and related sequences. Genomics 18: 105-112, 1993. PubMed: 8276393 Douglas A Andres, personal communication |