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| 产品名称： | lambdaEGF116 |
|---|---|
| 商品货号： | TS133799 |
| Designations： | lambdaEGF116 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 47.79999923706055 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.5 Tissue: adult kidney Gene product: epidermal growth factor(urogastrone) EGF Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 4.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--18.9, 7.4, 7.2, 5.2, 3.6, 1.4; EcoRI--30.7, 12.1, 4.4; HindIII--24.3, 9.9, 7.6, 4.4, 4.0; BglII--23.1, 12.5, 11.4, 4.7; SmaI--20.9, 8.9, 8.5, 6.9, 5.5. A 6.4 kb SmaI fragment of this construct (62 bp 5 untranslated, the coding sequence, through 2.0 kb of the right arm of lambdagt10) was inserted into pSAD and transfected into COS-7 cells. Immunoreactive membrane protein was detected. The insert includes nucleotides 328-4848 of the composite cDNA sequence, encompassing about 115 bp 5 untranslated, the complete coding sequence of 3621 bp, and 791 bp of 3 untranslated sequence. |
| References： | Bell GI, et al. Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic Acids Res. 14: 8427-8446, 1986. PubMed: 3491360 |