宁波泰斯拓生物

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cDNA 210

货号 TS133803
中文名称 null
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产品名称: cDNA 210
商品货号: TS133803
Designations: cDNA 210
GenBank Number:

X63546

Species: Homo sapiens, human
Depositors: M Hill
Applications:
The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe.
Vector:
Construct size (kb): 9.300000190734863
Insert:
DNA: cDNA
Insert lengths(kb): 6.300000190734863
Tissue: DNA from ED1 cell line (tre-transfected NIH 3T3)
Gene product: tre-2 oncogene(tre-2 oncogene) TRE2*
Insert Size (kb): 6.300
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.1, 2.4, 0.46; PstI--7.6, 1.45, 0.72; HindIII--5.2, 4.5; PvuII--2.6, 2.5, 1.10, 0.82, 0.74 (doublet), 0.49 (doublet); SalI--9.6.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.41, 3.96; PstI--0.67, 2.10; HindIII--1.92.
Contains two complete open reading frames, from nt 112-2472 and from nt 2460-4220 of the insert.
The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. It detects mRNA of 8.2 kb in Ewings sarcoma cells (the source of the transfected DNA) and other neoplasms.
Corresponds to nt 1586 - 7878 of the sequence record.
References:

Nakamura T, et al. A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene 7: 733-741, 1992. PubMed: 1565468

Nakamura T, et al. Nucleotide sequence of mouse L19 ribosomal protein cDNA isolated in screening with tre oncogene probes. DNA Cell Biol. 9: 697-703, 1990. PubMed: 1702292