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| 产品名称： | pSGP573 |
|---|---|
| 商品货号： | TS133831 |
| Designations： | pSGP573 |
| Depositors： | J Hodson |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Intact vector size: 9.300
Type of vector: plasmid
Cloning sites: XhoI SalI BamHI SmaI Polylinker sites: XhoI SalI BamHI SmaI Cloning capacity: Y Construction: REP4X Host range: Schizosaccharomyces pombe; Escherichia coli Features (with orientation and position when available): coding sequence: GFP, -> replicon: ars1 marker(s): ampR marker(s): ura4+ promoter for expression: nmt, -> MCS: XhoI...SmaI, -> Vector: pSGP573 (plasmid)
Promoters: Promoter for expression nmt
Construction: REP4X
Marker(s): ampR
Construct size (kb): 9.30
Features: marker(s): ampR marker(s): ura4+ promoter for expression: nmt replicon: ars1 MCS: XhoI...SmaI coding sequence: GFP |
| Applications： | contains sequence green fluorescent protein (GFP) |
| Comments： | GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 7-720 of GFP matching nucleotides 21-734 of U87624, with additional modification S65T by changing codon to ACC. The order of the major features in this plasmid is: nmt promotor - XhoI - ATG - GFP - GCT - NotI - SalI - BglII - SmaI - termination sequence - ars1 - pUC119 (3.3 kb) - ura4 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| Shipped： | freeze-dried |