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| 产品名称： | pBSL175 |
|---|---|
| 商品货号： | TS133863 |
| Designations： | pBSL175 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.2519998550415040 Vector: pBSL175 (plasmid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pSU18 Marker(s):cmlR,spcR,strR Construct size (kb): 4.251999855041504 Features: marker(s): cmlR promoter for in vitro transcription: T7 (phi10) replicon: p15A MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: aadA (spcR, strR) |
| Applications： | contains easily purifiable cassette(s) for construction omega-aadA1 |
| Comments： | Restriction digests of the clone give the following sizes (kb): MluI--2.2, 2.1; EcoRI--2.2, 2.1; BamHI--2.2, 2.1. Plasmid useful for vector construction. Contains an omega-Gm element (aadA, strR, spcR) flanked by multiple cloning sites duplicated in tandem orientation. |
| Media： | ATCC® Medium 1632: LB medium (ATCC medium 1065) with 10 mcg/ml chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | frozen |