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| 产品名称： | pBamtheta |
|---|---|
| 商品货号： | TS133884 |
| Designations： | pBamtheta |
| Species： | Homo sapiens, human |
| Depositors： | DR Higgs |
| Vector： | Construct size (kb): 3.769999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8299999833106995 Tissue: placenta Gene product: hemoglobin, theta 1 HBQ1 |
| Insert Size (kb)： | 0.830 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains the following sites separated by (bp)(approx): BamHI- 540- PvuII- 180- BstEII- 110- BamHI. Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.77; PvuII--3.1, 0.61; BstEII--3.8; HindIII/EcoRI--2.9, 0.84; HindIII--3.8. Detects a single copy sequence at high stringency, but detects pseudogenes at low stringency. pBamtheta is the 830 bp BamHI fragment of calpha3pSVED of E. Whitelaw. (calpha3pSVED is the 2.5 kb EcoRI/HindIII fragment of calpha3Bg cloned into the ScaI site of pSVED.). |
| References： | Hsu SL, et al. Structure and expression of the human theta-1 globin gene. Nature 331: 94-96, 1988. PubMed: 3422341 Douglas R Higgs, personal communication |