产品名称: | pBS-MCAV |
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商品货号: | TS133929 |
Designations: | pBS-MCAV |
GenBank Number: | X51791 |
Species: | Mus musculus, mouse |
Depositors: | WS Sly |
Vector: | Construct size (kb): 4.159999847412109 |
Insert: | DNA: cDNA Insert lengths(kb): 1.161999940872192 Tissue: liver Gene product: carbonic anhydrase-5, mitochondrial Car5 |
Insert Size (kb): | 1.162 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): XhoI--4.2; XbaI/XhoI--3.0, 1.2; EcoRI--4.2; SacI--3.6, 0.58; EcoRI/XhoI--3.6, 0.7. The insert contains the following restriction sites (approximate kb from the 5 end): AccI--1.18; EcoRI--0.57; SacI--0.55. The sequence of this insert differs from that of X51791 by having 3 additional nucleotides: X51791, nt 561-570: gttcatgaat, and this insert, 561-573: ggttcattggaat. |
Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
References: | Nagao Y, et al. Mitochondrial carbonic anhydrase (isozyme V) in mouse and rat: cDNA cloning, expression, subcellular localization, processing, and tissue distribution. Proc. Natl. Acad. Sci. USA 91: 10330-10334, 1994. PubMed: 7937950 William S Sly, personal communication |