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微信小章| 产品名称： | 2A1 Blast 1 cDNA, pB2A1, pB2A1.12 |
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| 商品货号： | TS133967 |
| Designations： | 2A1 Blast 1 cDNA, pB2A1, pB2A1.12 |
| GenBank Number： | X06341 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Tissue: lymphoblastoid cell line immortalized with EBV Gene product: CD48 antigen(antigen CD48 (B cell membrane protein)) BCM1 Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 1.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Insert contains SacI (3), SphI (1), and HincII (1) sites. Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; HincII--4.2, plus smaller; HindIII--4.4; PstI--4.4; BamHI--4.4. The protein resulting from in vitro translation of this cDNA (unglycosylated, with signal peptide) is precipitated by MAb 17D6 specific to Blast-1. Detects human genomic restriction fragments of the following sizes (kb): EcoRI--9.3, 6.7, 3.4 (weak); HindIII--2.9, 2.1, 1.2, 0.8. This is a full-length or close to full-length cDNA clone including 46 bp 5 untranslated and about 430 bp 3 untranslated sequence. The sequence predicts 5 N-glycosylation sites. The insert is oriented such that transcription from the T7 promoter gives the sense strand. |
| References： | Lai LW, et al. A gene correcting the defect in the CHO mutant Ade-H, deficient in a branch point enzyme (adenylosuccinate synthetase) of de novo purine biosynthesis, is located on the long arm of chromosome 1. Genomics 9: 322-328, 1991. PubMed: 2004783 Fisher RC, Thorley-Lawson DA. Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48). Mol. Cell. Biol. 11: 1614-1623, 1991. PubMed: 1847502 Staunton DE, Thorley-Lawson DA. Molecular cloning of the lymphocyte activation marker Blast-1. EMBO J. 6: 3695-3701, 1987. PubMed: 2828034 David A Thorley-Lawson, personal communication |