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| 产品名称： | pUCM11 |
|---|---|
| 商品货号： | TS134092 |
| Designations： | pUCM11 |
| GenBank Number： | M14211 |
| Species： | Homo sapiens, human |
| Applications： | produces protein ferritin, heavy polypeptide 1 Ferritin subunit H |
| Vector： | Construct size (kb): 9.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 7.199999809265137 Tissue: liver Gene product: ferritin, heavy polypeptide 1(Ferritin subunit H) FTH1 Target Gene: ferritin, heavy polypeptide 1 |
| Insert Size (kb)： | 7.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--7.0, 2.7; BamHI--4.3, 2.8, 2.5; EcoRI--6.6, 3.3; PstI--4.0, 2.8, 1.3, 0.8, 0.7, 0.5; SalI--9.8. The insert contains the entire coding sequence (4 exons) plus upstream and downstream sequences. The EcoRI fragment in the insert is smaller than the genomic sequence it detects, suggesting truncation during construction. Only a partial sequence of promoter, intron I, all exons and introns II and III is available. Insert is expressed in transformed murine B6 fibroblasts. A 0.7 kb PstI fragment which is upstream of the TATA sequence is unique and was used to map the gene. It does not cross-hybridize with hamster or mouse DNA. |
| References： | Hentze MW, et al. Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene. Proc. Natl. Acad. Sci. USA 83: 7226-7230, 1986. PubMed: 3020541 Hentze MW, et al. A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron. Proc. Natl. Acad. Sci. USA 84: 6730-6734, 1987. PubMed: 3477805 |