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| 产品名称： | lambda2 GC.2 |
|---|---|
| 商品货号： | TS134134 |
| Designations： | lambda2 GC.2 |
| GenBank Number： | L10641 |
| Species： | Homo sapiens, human |
| Depositors： | A Dugaiczyk |
| Vector： | Construct size (kb): 49.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: Charon 4A Intact vector size: 46.000 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): enhancer: none promoter: none replicon: lambda terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GC Genomic copy number: unique Gene name: group-specific component (vitamin D binding protein) Contains complete coding sequence?: N Chromosome: 4; Localization: 4 q12-q13 Tissue: fetal liver (ATCC 37333) Type of DNA: genomic Insert end: HaeIII or AluI Modification: EcoRI linker Insert end: HaeIII or AluI Modification: EcoRI linker Insert size (kb): 18.0 Cross references: DNA Seq. Acc.: L10641 Nucleotides ~1-18000 of the insert correspond to nucleotides ~37000-55136 of L10641. Insert lengths(kb): 18.0 Tissue: fetal liver (ATCC 37333) Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) GC |
| Insert Size (kb)： | 18.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 9.8, 3.5, 3.3, 1.4; HindIII--19, 14, 6, 4.4, 4, 2, 0.4; BamHI--17, 11, 7.5, 5.8, 3.9, 3, 1.6, 0.5; BglII-->23, 5.8, 5, 2.15, 1.95, 1.9, 1.8, 1.4, 0.7, 0.4; SstI-->23, 15, 4, 3.8, 1.9. The insert contains the following restriction site (approximate kb from the 5 end): EcoRI--1.4, 4.7, 14.5; HindIII--6.6, 11.0, 15.0, 15.4, 17.4; SacI--14.6, 18.8. Begins in intron 11 and extends into the 3 flanking region. ATCC 95668/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb. Contains Alu repeats, L1 (Kpn) repeats, and a THE repeat. |
| References： | Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650 |