| 产品名称: | p4D8-B p4D8B |
|---|---|
| 商品货号: | TS134180 |
| Designations: | p4D8-B p4D8B |
| Species: | Homo sapiens, human |
| Depositors: | RE Schnur |
| Applications: | This insert contains some repetitive sequences and requires preannealing when used for Southern blot hybridizations. |
| Vector: | Construct size (kb): 10.69999980926514 |
| Insert: | DNA: genomic Insert lengths(kb): 8.0 Tissue: lymphoblast GM1416 (48,XXXX) cell line Gene product: DNA Segment, single copy DXS98 Alleles: A1, B1, B2, C1, C2, D2, D1, A2 |
| Insert Size (kb): | 8.0 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--7.2, 2.4, 1.1; HindIII--4.2, 3.4, 3.1; BamHI--10.9; PstI--4.1, 3.5, 1.4, 1.0, 0.7; SalI/BamHI--8.0, 2.9. DXS98 shows close linkage to FRAXA (theta = 0.10, LOD score = 17.31). DXS98 is proximal to FRAXA by in situ hybridization. Combined heterozygosity for the polymorphisms detected by 4D-8 (ATCC 57060, 57061), p4D8-B (TS134180, 61573), and p4D8-IV (ATCC 61574, 61575) is 48%. This insert contains some repetitive sequences and requires preannealing when used for Southern blot hybridizations. |
| References: | Schnur RE, et al. New polymorphisms at the DXS98 locus and confirmation of its location proximal to FRAXA by in situ hybridization. Am. J. Hum. Genet. 44: 248-254, 1989. PubMed: 2563194 |