宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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p4D8-B [p4D8B]

货号 TS134180
中文名称 null
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产品名称: p4D8-B p4D8B
商品货号: TS134180
Designations: p4D8-B p4D8B
Species: Homo sapiens, human
Depositors: RE Schnur
Applications:
This insert contains some repetitive sequences and requires preannealing when used for Southern blot hybridizations.
Vector:
Construct size (kb): 10.69999980926514
Insert:
DNA: genomic
Insert lengths(kb): 8.0
Tissue: lymphoblast GM1416 (48,XXXX) cell line
Gene product: DNA Segment, single copy DXS98
Alleles: A1, B1, B2, C1, C2, D2, D1, A2
Insert Size (kb): 8.0
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2, 2.4, 1.1; HindIII--4.2, 3.4, 3.1; BamHI--10.9; PstI--4.1, 3.5, 1.4, 1.0, 0.7; SalI/BamHI--8.0, 2.9.
DXS98 shows close linkage to FRAXA (theta = 0.10, LOD score = 17.31).
DXS98 is proximal to FRAXA by in situ hybridization.
Combined heterozygosity for the polymorphisms detected by 4D-8 (ATCC 57060, 57061), p4D8-B (TS134180, 61573), and p4D8-IV (ATCC 61574, 61575) is 48%.
This insert contains some repetitive sequences and requires preannealing when used for Southern blot hybridizations.
References:

Schnur RE, et al. New polymorphisms at the DXS98 locus and confirmation of its location proximal to FRAXA by in situ hybridization. Am. J. Hum. Genet. 44: 248-254, 1989. PubMed: 2563194