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微信小章| 产品名称： | pERT84-10 |
|---|---|
| 商品货号： | TS134191 |
| Designations： | pERT84-10 |
| Species： | Homo sapiens, human |
| Depositors： | LM Kunkel |
| Vector： | Construct size (kb): 6.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 2.5 Gene product: DNA Segment, single copy (within DMD gene) DXS142 Alleles: A1, A2 |
| Insert Size (kb)： | 2.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.8, 0.8; EcoRI--6.9; PstI--6.9; BamHI--4.4, 2.5; SalI--6.9. This clone is at the 5 most end of the DMD locus. HindIII digestion yields 2 fragments from the insert: 1.8 kb and 0.7 kb. The larger fragment recognizes the two constant TaqI bands of 3.4 and 1.75 kb. The 0.7 kb HindIII fragment detects the TaqI RFLP. The polymorphisms detected by this probe are contained within the DMD locus. |
| References： | Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877 Bertelson CJ, et al. Localisation of Xp21 meiotic exchange points in Duchenne muscular dystrophy families. J. Med. Genet. 23: 531-537, 1986. PubMed: 2879924 Kunkel LM, et al. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion. Proc. Natl. Acad. Sci. USA 82: 4778-4782, 1985. PubMed: 2991893 Louis M Kunkel, personal communication |