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| 产品名称： | pOSEX2 |
|---|---|
| 商品货号： | TS134210 |
| Designations： | pOSEX2 |
| Depositors： | E Bremer |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli MKH13 (ATCC 47087) |
| Vector Information： | Size (kb): 5.3800001144409180 DESCRIPTION OF VECTOR: Intact vector size: 5.380 Type of vector: plasmid Cloning sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI SacI SphI EcoRV NcoI HindIII Polylinker sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI SacI PstI SphI EcoRV NcoI StyI HindIII Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR, ->, 84-1274 replicon: rop (copy number control), 1913-2104 replicon: pMB1, 2532-2532 marker(s): ampR, transcription terminator: rrnB T2, transcription terminator: rrnB T1, MCS: BamHI...HindIII, coding sequence: proV 5 sequence, promoter for expression: proU, Vector: pOSEX2 (plasmid) Promoters: Promoter for expression proU Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322 Marker(s):ampR,tetR Construct size (kb): 5.380000114440918 Features: marker(s): ampR marker(s): tetR promoter for expression: proU replicon: pMB1 replicon: rop (copy number control) MCS: BamHI...HindIII transcription terminator: rrnB T1 transcription terminator: rrnB T2 coding sequence: proV 5 sequence |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.05; HindIII--4.6, 0.8; PvuII--5.4. Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride. Vector does not provide a ribosome binding site or initiation codon. Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter. This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862 Erhard Bremer, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |