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微信小章| 产品名称： | (S)CARNA5(-) |
|---|---|
| 商品货号： | TS134298 |
| Designations： | (S)CARNA5(-) |
| GenBank Number： | M14934 |
| Species： | Cucumber mosaic virus |
| Depositors： | JM Kaper |
| Applications： | Oriented such that transcription from the SP6 promoter gives the antisense (-) strand, that can be used as a probe to detect satellite sequences. |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.4020000100135803 Gene product: satellite RNA (CMV-associated RNA 5, CARNA 5) |
| Insert Size (kb)： | 0.402 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.4; HindIII--3.4; BamHI--3.4; PstI--3.4; XbaI--3.4. Contains a BstEII site 68 nt from the 5 end. A full-length clone. Oriented such that transcription from the SP6 promoter gives the antisense (-) strand, that can be used as a probe to detect satellite sequences. |
| Classification： | Cucumovirus |
| References： | Avila-Rincon MJ, et al. In vitro translation of cucumoviral satellites. I. Purification and nucleotide sequence of a cucumber mosaic virus-associated RNA 5 from cucumber mosaic virus strain S. Virology 152: 446-454, 1986. Avila-Rincon MJ, et al. In vitro translation of cucumoviral satellites. II. CARNA 5 from cucumber mosaic virus strain S and SP6 transcripts of cloned (S)CARNA 5 cDNA produce electrophoretically comigrating protein products. Virology 152: 455-458, 1986. PubMed: 3487880 Steen MT, et al. In-vitro translation of cucumoviral satellites. III. Translational efficiencies of cucumber mosaic virus-associated RNA 5 sequence variants can be related to the predicted secondary structures of their first 55 nucleotides. Virus Genes 4: 41-52, 1990. PubMed: 1697436 J M Kaper, personal communication |