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| 产品名称： | pCR1000DQB1*0607 |
|---|---|
| 商品货号： | TS134316 |
| Designations： | pCR1000DQB1*0607 |
| GenBank Number： | M87041 |
| Species： | Homo sapiens, human |
| Depositors： | L Baxter-Lowe |
| Vector： | Construct size (kb): 3.269999980926514 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCR1000 Intact vector size: 3.000 Type of vector: plasmid Vector end: T overhangs Vector end: T overhangs Cloning sites: Polylinker sites: ApaI KpnI SacI SpeI PacI HindIII XmaI ins site NotI EcoRI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): kanR promoter: lac, T7 replicon: colE1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HLA-DQB1 Other gene symbols: HLA-DQB Genomic copy number: unique Gene name: major histocompatibility complex, class II, DQ beta 1 Contains complete coding sequence?: U Chromosome: 6; Localization: 6 p21.3 Tissue: peripheral blood leukocytes Type of DNA: genomic Insert end: Modification: A overhangs Insert end: Modification: A overhangs Insert size (kb): 0.27 Cross references: DNA Seq. Acc.: M87041 Insert lengths(kb): 0.2700000107288361 Tissue: peripheral blood leukocytes Gene product: major histocompatibility complex, class II, DQ beta 1(major histocompatibility complex, class II, lymphocyte antigen DQB) HLA-DQB |
| Insert Size (kb)： | 0.270 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--2.9, 0.38; SmaI--2.8, 0.4, 0.2; BamHI--uncut; EcoRI/HindIII--3.0, 0.32; HindIII--3.3. The insert contains the following restriction sites (bp from the 5 end): BglI--129; SmaI--188. Encodes the *0607 or the *06BRI1 allele, which differs from DQB1*0603 by a substitution at codon 57 GTT (valine) replacing GAT (aspartic acid). |
| References： | Fenske TS, Baxter-Lowe LA. Two novel HLA-DQB1*06 alleles reveal additional heterogeneity of HLA-DQw1. Tissue Antigens 40: 49-52, 1992. PubMed: 1440562 |