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微信小章| 产品名称： | pRed-227 |
|---|---|
| 商品货号： | TS134322 |
| Designations： | pRed-227 |
| Species： | Cricetus cricetus, hamster |
| Depositors： | KL Luskey |
| Vector： | Construct size (kb): 7.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.5 Tissue: CHO UT1 cell line Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase HMGCR |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--7.4; BamHI--4.37, 2.7, 0.3. A full-length clone which includes the entire coding region, 163 bp of 5-untranslated sequence and 1.65 kb of 3-untranslated sequence. It codes for a protein of 887 amino acids. Hybridizes to genomic EcoRI fragments of the following sizes: mouse 3T3 (14.5, 2.1 kb), Chinese hamster (10.1, 3.3, 2.8, 1.45 kb), and rat (9.1, 7.7, 3.2 kb). |
| References： | Simonet WS, Ness GC. Post-transcriptional regulation of 3-hydroxy-3-methylglutaryl-CoA reductase mRNA in rat liver. Glucocorticoids bloc the stabilization caused by thyroid hormones. J. Biol. Chem. 264: 569-573, 1989. PubMed: 2909540 Simonet WS, Ness GC. Transcriptional and posttranscriptional regulation of rat hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase by thyroid hormones. J. Biol. Chem. 263: 12448-12453, 1988. PubMed: 3410847 Sundaresan S, et al. Genes for HMG-CoA reductase and serotonin 1a receptor are on mouse chromosome 13. Somatic Cell Mol. Genet. 15: 465-469, 1989. PubMed: 2781417 Chin DJ, et al. Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum. Nature 308: 613-617, 1984. PubMed: 6546784 |