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| 产品名称： | pBD64 |
|---|---|
| 商品货号： | TS134345 |
| Designations： | pBD64 |
| Depositors： | D Dubnau |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.6999998092651370 Vector: pBD64 (plasmid) Construction: pUB110, pC194 Marker(s):cmlR,kanR Construct size (kb): 4.699999809265137 Features: marker(s): cmlR, kanR replicon: pUB110 |
| Applications： | shuttle vector |
| Comments： | A general purpose plasmid vector for cloning in Bacillus subtilis. This vector was isolated as a spontaneous deletion variant of pBD12, a chimeric vector composed of plasmids pUB110 and pC194. The deletion consists of 1844 bp (nt 3803 to 5649) of pBD12, and encompasses nt 1 to 487 and 1552 to 2906 of the pC194 sequence. |
| Media： | ATCC® Medium 1179: VY medium |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622 Minton NP, et al. The gram-positive cloning vector pBD64 arose by a 1844 bp deletion of pC194 derived DNA. Nucleic Acids Res. 18: 1651, 1990. PubMed: 2326208 |
| Shipped： | freeze-dried |