产品名称: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
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商品货号: | TS134353 |
Deposited As: | Saccharomyces cerevisiae Hansen, teleomorph |
Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
Strain Designations: | BY4718 |
Alternate State: | Candida robusta Diddens et Lodder |
Application: | transformation host with non-revertable mutations for auxotrophic markers transformation host with non-revertable designer deletions |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format: | frozen |
Type Strain: | no |
Genotype: | MATa met15delta0 ura3delta0
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Preceptrol®: | no |
Mating Type: | a |
Ploidy: | haploid |
Comments: | One of a series of strains (ATCC 200866-200902) useful for gene disruption experiments. The strains differ in mating type and in non-revertable mutations for one or more of these auxotrophic markers: ade2delta::hisG, his3delta200, leu2delta0, lys2delta0, met15delta0, trp1delta63, ura3delta0. |
Morphology: | After 3 days on YEPD medium at 25°C, colonies are off-white, smooth, butyrous. Cells are ovoid or irregular; single, in pairs or small clusters. |
Medium: | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
Growth Conditions: | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
Sequenced Data: |
No DNA sequencing was performed in house on this product.
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Name of Depositor: | JD Boeke |
Special Collection: | NCRR Contract |
References: | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |