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| 产品名称： | HGR3.5 HUMGRA2 |
|---|---|
| 商品货号： | TS134511 |
| Designations： | HGR3.5 HUMGRA2 |
| GenBank Number： | M73816 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.900000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRL Genomic copy number: unique Gene name: glucocorticoid receptor Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q31-q32 Tissue: leukocyte Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 3.5 Cross references: DNA Seq. Acc.: M73816 Nucleotides 1-1628 of the insert correspond to nucleotides 1-1628 of M73816 with 100% identity. Insert lengths(kb): 3.5 Tissue: leukocyte Gene product: glucocorticoid receptor GRL |
| Insert Size (kb)： | 3.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--3.3, 2.4, 2.0, 0.26; EcoRI--4.4, 3.4; HindIII--4.8, 1.1, 0.68, 0.60, 0.50, 0.30; PvuII--3.9, 2.4, 1.8; SalI--6.5, 1.6. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.93; BglII--0.64, 1.20; HindIII--0.14, 0.77, 1.34; PvuII--0.16; SalI--0.81. Contains part of intron 1, exon 2 and part of intron 2. Subcloned from lambdaGHGR-5. ATCC 80008/9 and ATCC 80010/11 are contiguous in the genome. |
| References： | Govindan MV, et al. Human glucocorticoid receptor gene promoter-homologous down regulation. J. Steroid Biochem. Mol. Biol. 40: 317-323, 1991. PubMed: 1958537 Leclerc S, et al. Purification of a human glucocorticoid receptor gene promoter-binding protein. J. Biol. Chem. 266: 8711-8719, 1991. PubMed: 2026589 |