| 产品名称： |
Trypanosoma cyclops Weinman |
| 商品货号： |
TS134514 |
| Deposited As： |
Trypanosoma conorrhini (Donovan) Shortt and Swaminath |
| Strain Designations： |
Malacca |
| Application： |
Vector borne research |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： |
Kissing bug, Triatoma rubrofasciata, Malacca, Malaysia, 1974 |
| Product Format： |
frozen |
| Storage Conditions： |
Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures:
2-8°C
Live Cultures:
See Protocols section for handling information |
| Type Strain： |
no |
| Medium： |
ATCC® Medium 431: Trypanosome medium
|
| Growth Conditions： |
Temperature: 25°C
Culture System: Axenic |
| Cryopreservation： |
Harvest and Preservation
- Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
- Adjust concentration of cells to 0.5–1.0 x 107/mL in fresh growth medium. xa0If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
- While cells are centrifuging, prepare a 20% (v/v) solution of sterile glycerol in fresh growth medium.xa0
- Mix the cell preparation and the glycerol solution in equal portions. The final concentration will be 2.5-xad5 x 106xa0cells/mL in 10% glycerol. The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
- Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screwxadcapped cryovials.
- Place the ampules in a Nalgene 1°C freezing apparatus. xa0Place the apparatus at xad-80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. xa0(The cooling rate in this apparatus is approximately -xad1°C/min.) xa0If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. xa0At -40°C, plunge ampules into liquid nitrogen.
- Store in either the vapor or liquid phase of a nitrogen refrigerator.
- To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. xa0Do not agitate the ampule. xa0Do not leave ampule in water bath after it is thawed.
- Remove the vial from the water bath immediately after thawing. xa0Aseptically transfer the contents of the ampule into a 16 x 125 mm glass screw-capped test tube of fresh growth medium.xa0
- Incubate the test tube vertically at 25°C with the cap screwed on tightly.
- Maintain as described above.xa0
|
| Name of Depositor： |
D Weinman |
| Year of Origin： |
1974 |
微信小陈
微信小章