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| 产品名称： | RR2 |
|---|---|
| 商品货号： | TS134573 |
| Designations： | RR2 |
| Depositors： | DH Jones |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 1.9140000343322750 Vector: RR2 (plasmid) Construction: pUC19 Marker(s):ampR Construct size (kb): 1.914000034332275 Features: marker(s): ampR replicon: pMB1 MCS: BssHII...NotI |
| Applications： | vector useful for site-directed mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--1.9; BamHI--1.9; XhoI--1.9. Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; FspI--1.28; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05. Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts. Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker. Differs from RR1 (ATCC 87076) and GenBank S70522 by the presence of a unique FspI site (T1280G) and a C1100T transition. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jones DH, et al. Production of a vector to facilitate DNA mutagenesis and recombination. BioTechniques 16: 694-701, 1994. PubMed: 8024792 |
| Shipped： | freeze-dried |