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| 产品名称： | pLGdelta312 |
|---|---|
| 商品货号： | TS134632 |
| Designations： | pLGdelta312 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.1999998092651400 Vector: pLGdelta312 (plasmid) Promoters: Promoter CYC1 promoter (including the CYC1 UAS) Construction: pBR322 Marker(s):URA3,ampR Construct size (kb): 10.19999980926514 Features: marker(s): URA3 marker(s): ampR promoter: CYC1 promoter (including the CYC1 UAS) replicon: 2 micron ori replicon: pMB1 coding sequence: lacZ |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--10.3; HindIII--8.0, 2.1; EcoRI--4.5, 3.15, 2.25. This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (TS134632, 87512 & 87522). They differ by polylinker sites. DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity. The vector was constructed by Lenny Guarente. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Guarente L, Hoar E. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA" box. Proc. Natl. Acad. Sci. USA 81: 7860-7864, 1984. PubMed: 6096863 |
| Related Products： | component of:ATCC 87540 |
| Shipped： | freeze-dried |