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| 产品名称： | pSV2-neo plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS134634 |
| Designations： | pSV2-neo plasmid in Escherichia coli |
| Depositors： | P Berg, Stanford University Medical Center |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 5.729
Vector: pSV2-neo (plasmid)
Promoters: Promoter SV40 early
Construction: SV40, Tn5, pBR322
Marker(s): G418R,ampR
Construct size (kb): 5.729
Features: marker(s): G418R marker(s): ampR promoter: SV40 early replicon: SV40 replicon: pMB1 restriction site: HindIII |
| Applications： | Expression vector
Shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet). Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831 |
| Shipped： | frozen |
| Shipping Information： | Glycerol stock of xa0E. coli containing the plasmid |