宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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lambdaF6

货号 TS134667
中文名称 null
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产品名称: lambdaF6
商品货号: TS134667
Designations: lambdaF6
GenBank Number:

M12949

Species: Homo sapiens, human
Vector:
Construct size (kb): 42.79999923706055
Insert:
DNA: genomic
Insert lengths(kb): 13.30000019073486
Tissue: F8 cell line (T-prolymphocytic leukemia)
Gene product: T cell receptor, gamma, variable region V3 TCRGJ1
Insert Size (kb): 13.300
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): KpnI--17.0, 11.0, 4.8, 3.6, 1.8 (doublet), 1.5; PvuI--22.0, 13.0, 7.8; SacI--20.0, 9.1, 4.4 (doublet), 4.1; SmaI--20.0, 11.0, 6.2, 5.8; XhoI--20.0, 14.0, 9.1.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--3.3, 5.1, 10.5.
Contains a productive rearrangement of Vgamma3 and Jgamma1. The insert begins about 0.5 kb upstream of pseudogene Vgamma1 and extends 3.6 kb beyond J1.
Additional GenBank/EMBL accession numbers: M12953, M13429.
References:

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication