产品名称: | lambdaF6 |
---|---|
商品货号: | TS134667 |
Designations: | lambdaF6 |
GenBank Number: | M12949 |
Species: | Homo sapiens, human |
Vector: | Construct size (kb): 42.79999923706055 |
Insert: | DNA: genomic Insert lengths(kb): 13.30000019073486 Tissue: F8 cell line (T-prolymphocytic leukemia) Gene product: T cell receptor, gamma, variable region V3 TCRGJ1 |
Insert Size (kb): | 13.300 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
Comments: | Restriction digests of the clone give the following sizes (kb): KpnI--17.0, 11.0, 4.8, 3.6, 1.8 (doublet), 1.5; PvuI--22.0, 13.0, 7.8; SacI--20.0, 9.1, 4.4 (doublet), 4.1; SmaI--20.0, 11.0, 6.2, 5.8; XhoI--20.0, 14.0, 9.1. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--3.3, 5.1, 10.5. Contains a productive rearrangement of Vgamma3 and Jgamma1. The insert begins about 0.5 kb upstream of pseudogene Vgamma1 and extends 3.6 kb beyond J1. Additional GenBank/EMBL accession numbers: M12953, M13429. |
References: | LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743 Marie-Paule Lefranc, personal communication |