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| 产品名称： | pHG4.5 |
|---|---|
| 商品货号： | TS134680 |
| Designations： | pHG4.5 |
| GenBank Number： | M61829 |
| Species： | Homo sapiens, human |
| Depositors： | H Schachter |
| Vector： | Construct size (kb): 7.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-5Zf(+) Intact vector size: 3.000 Type of vector: phagemid Vector end: NcoI Vector end: SalI Cloning sites: Polylinker sites: Features (with orientation and position when available): |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MGAT1 Other gene symbols: GLYT1; MGAT Gene name: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 Tissue: chronic myeloid leukemia Type of DNA: genomic Insert 5 end: Insert 3 end: Insert size (kb): 4.5 Cross references: DNA Seq. Acc.: M61829 Insert lengths(kb): 4.5 Tissue: chronic myeloid leukemia Gene product: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase MGAT |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NcoI/SalI--4.5, 3.0; EcoRI--7.5; PstI--4.25, 2.25, 0.9; SacI--4.4, 1.95, 0.65, 0.4; SmaI--7.5. The insert contains the following restriction sites (kb from the 5 end): BglI--3.03; BglII--3.1; EcoRI--4.33; PstI--2.2, 2.4, 3.23, 3.28; PvuII--3.21, 3.28, 4.17; SacI--2.43, 2.58, 3.0; SmaI--2.33. An 0.8 kb PstI fragment of the insert hybridizes to the following fragments of human genomic DNA (kb): EcoRI--4.7; HindIII--7.2; BamHI--10.4; BglII--4.0; StyI--1.3. Detects a 9.0 kb EcoRI fragment of hamster genomic DNA. Contains a complete exon 2 (2.5 kb) plus upstream and downstream sequences. Exon 2 contains most of the 5 untranslated region, the complete coding sequence (nt 2182-3519 of the sequence record) and the complete 3 untranslated region. Derived from pHG15 which was constructed by inserting a SalI fragment from an lambdaEMBL3 library into pGEM-5Z. pHG4.5 is a circularized NcoI fragment from pHG15, and thus retains lambdaEMBL3 sequences from SalI to the 5 end of the insert. The SP6 promoter is adjacent to the 5 end of the insert. |
| References： | Hull E, et al. Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene. Biochem. Biophys. Res. Commun. 176: 608-615, 1991. PubMed: 1827260 Harry Schachter, personal communication |