微信小陈
微信小章| 产品名称： | pCETP.11 CEPT.10, lambdaCEPT.10, pCETP.11A |
|---|---|
| 商品货号： | TS134697 |
| Designations： | pCETP.11 CEPT.10, lambdaCEPT.10, pCETP.11A |
| Species： | Homo sapiens, human |
| Depositors： | DT Drayna |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.580000042915344 Tissue: liver Gene product: cholesteryl ester transfer protein, plasma CETP Alleles: C1, C2, A1, S1, A2, B1, B2, E1, E2, S2 |
| Insert Size (kb)： | 1.580 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7; BglII--3.2, 1.1; PvuII--2.4, 1.0, 0.54, 0.36; NdeI--4.5; BglI--3.9, 0.47. Codominant segregation of both TaqI polymorphisms in 4 families (29 individuals). TaqIA and TaqIB polymorphisms exhibit a large degree of linkage equilibrium; no recombination between the two has been shown. Contains the CETP coding sequence from amino acid 9 of the mature protein through the 3 untranslated sequence, ending with 17 residues of polyA. Four haplotypes with frequencies 0.06 to 0.44. S1/S2 alleles are difficult to detect with the entire probe. A 732 bp PstI/PvuII fragment detects the same polymorphism and the 3.4 kb invariant band. TaqIA RFLP hybridizes much more strongly than the TaqIB RFLP. The MspI RFLP is quite sensitive to incomplete digestion. Enzyme(s) not detecting polymorphism: BclI, BglII, BstEII, EcoRI, EcoRV, HindIII, PstI, PvuII and SacI. Enzyme(s) not detecting polymorphism: ApaLI, Asp718, BglII, BsmI, HincII, NcoI, NdeI, PstI, SacI, ScaI, SphI, StuI (in 6 unrelated individuals). |
| References： | Drayna D, et al. Cloning and sequencing of human cholesteryl ester transfer protein cDNA. Nature 327: 632-634, 1987. PubMed: 3600759 Drayna D, Lawn R. Multiple RFLPs at the human cholesteryl ester transfer protein (CETP) locus. Nucleic Acids Res. 15: 4698, 1987. PubMed: 2884631 Lusis AJ, et al. Assignment of the human gene for cholesteryl ester transfer protein to chromosome 16q12-16q21. Genomics 1: 232-235, 1987. PubMed: 3446583 Freeman D, et al. An StuI RFLP at the human cholesteryl ester transfer protein (CETP) locus. Nucleic Acids Res. 17: 2880, 1989. PubMed: 2566155 Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986 Kinzler KW, Vogelstein B. EcoRI polymorphism within the GLI gene (chromosome 12q13.3-14.1). Nucleic Acids Res. 18: 2834, 1990. PubMed: 1971098 Dennis T Drayna, personal communication |