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| 产品名称： | FN771 |
|---|---|
| 商品货号： | TS134734 |
| Designations： | FN771 |
| GenBank Number： | M10905 |
| Species： | Homo sapiens, human |
| Depositors： | M Chu |
| Applications： | The coding sequence includes part of the fibrin binding domain, the 3 region showing species-specific variation, and a conserved cysteine. |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.299999952316284 Tissue: fibroblast ATCC CRL 1106 cell line Gene product: fibronectin 1 FN1 Alleles: A2, E2, A1, B2, D2, B1, D1, E1 |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (number): EcoRI (1), HindIII (1), HaeII (1). Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.0, 0.3; HindIII--5.7; PstI--5.7; EcoRI/HindIII--4.3, 0.9, 0.3, 0.2; BamHI--5.7. The insert encodes the 3 500 bp of the coding sequence and 3 untranslated sequences. The coding sequence includes part of the fibrin binding domain, the 3 region showing species-specific variation, and a conserved cysteine. This probe detects a mRNA of 7.8 kb in human fibroblasts. |
| References： | Bernard MP, et al. Human cellular fibronectin: comparison of the carboxyl-terminal portion with rat identifies primary structural domains separated by hypervariable regions. Biochemistry 24: 2698-2704, 1985. PubMed: 2992573 |