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| 产品名称： | VC85 |
|---|---|
| 商品货号： | TS134743 |
| Designations： | VC85 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 2.099999904632568 Gene product: DNA Segment, single copy D1S85 Alleles: A1, A2 |
| Insert Size (kb)： | 2.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.1; BamHI--4.8; PstI--3.3, 1.5; SmaI--4.8; HindIII--4.8. D1S85 has been shown to be tightly linked to D1S86. This probe is a subclone of the 16.41 kb probe VC85. The 2.1 kb sequence in the insert is from the smaller EcoRI allele. The other allele is 2.6 kb. The allele frequencies given were determined in a Caucasian population. Distinct frequencies have been determined in Negro and Bushman (San) populations. This probe detects a 500 bp insertion/deletion polymorphism which can be detected with BglII, StuI, PvuII, PstI, EcoRI, HindIII, and BamHI. Because TaqI and MspI cut within this fragment, allele sizes do not vary by 500 bp. Enzyme(s) not detecting polymorphism: HindII, SstI, AvaII, RsaI, MboI. |
| References： | Verga V, et al. An arbitrary single copy DNA sequence VC85 (D1S85) detects a 500 bp insertion/deletion polymorphism on chromosome 1. Nucleic Acids Res. 17: 4007, 1989. PubMed: 2567507 Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294 Vera Verga, personal communication |