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| 产品名称： | cos203 |
|---|---|
| 商品货号： | TS134745 |
| Designations： | cos203 |
| Depositors： | D Kioussis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.1999998092651400 Vector: cos203 (cosmid) Promoters: Promoter HSV TK Marker(s):ampR,hygR Construct size (kb): 10.19999980926514 Features: marker(s): ampR, hygR promoter: HSV TK replicon: pMB1 terminator: none enhancer: none |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--10.05; XhoI--10.05; BglII--uncut. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The hygromycin resistance gene is expressed under the control of the HSV-TK promoter with a polyadenylation signal. This is not an expression vector. The cloning sites were derived from the M13mp10/pUC12 polylinker with the EcoRI site having been destroyed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Kioussis D, et al. Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV-based shuttle cosmid vector. EMBO J. 6: 355-361, 1987. PubMed: 3034585 Dimitris Kioussis, personal communication |
| Shipped： | freeze-dried |