产品名称: | CRI-L1020 |
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商品货号: | TS134832 |
Designations: | CRI-L1020 |
Species: | Homo sapiens, human |
Vector: | Construct size (kb): 49.5 |
Insert: | DNA: genomic Insert lengths(kb): 19.0 Tissue: liver Gene product: DNA Segment, single copy D7S62 Alleles: B4, A1, B2, A2, B1, B3, B5, B7, B6 |
Insert Size (kb): | 19.0 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--22.0, 13.0, 5.4 (doublet), 4.1, 3.2, 1.35; BamHI--21.0, 11.0, 8.2, 6.0, 4.1, 1.4; HindIII--22.0, 12.0, 8.0, 6.0, 3.5, 3.0, 1.9, 1.4, 0.88; BglII--22.0, 8.0, 6.2, 5.0, 3.9, 1.9, 1.6, 0.58; PvuI--23.0, 13.0, 8.0, 5.0, 2.5, 2.0. The HindIII and TaqI polymorphisms have a combined PIC value of 0.76 and a combined heterozygosity of 79%. Enzyme(s) not detecting polymorphism: MspI, PstI, BamHI, RsaI (panel of 5 individuals). |
References: | Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396 Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638 Kazazian HH Jr., et al. Characterization of a spontaneous mutation to a B-thalassemia allele. Am. J. Hum. Genet. 38: 860-867, 1986. PubMed: 3014870 Knowlton RG, et al. Use of highly polymorphic DNA probes for genotypic analysis following bone marrow transplantation. Blood 68: 378-385, 1986. PubMed: 3524708 Barker D, et al. Genetic linkage map of human chromosome 7 with 63 DNA markers. Proc. Natl. Acad. Sci. USA 84: 8006-8010, 1987. PubMed: 2891136 Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474 |