宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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Plasmodium falciparum Welch

货号 TS134843
中文名称 null
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产品简介
购买须知
产品名称: Plasmodium falciparum Welch
商品货号: TS134843
Strain Designations: HB-2
Application:
Emerging infectious disease research
Vector borne research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
derived from existing strain
Isolation date: 1981
Product Format: frozen
Storage Conditions: Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain: no
Comments:
This is a non-gametocyte-forming strain.
Medium: ATCC® Medium 2196: Malaria medium, complete
Growth Conditions:
Temperature: 37°C
Cryopreservation:

Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

  1. Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screwtop freezing vials and store at -80°C overnight.
  6. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.

** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:xa0 57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4°C.

Name of Depositor: W Trager
Special Collection: ATCC
Year of Origin: 1981
References:

Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092