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| 产品名称： | pRSVlacZII |
|---|---|
| 商品货号： | TS134881 |
| Designations： | pRSVlacZII |
| Depositors： | LA Culp |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 9.0000000000000000 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pRc/RSV Intact vector size: 5.100 Type of vector: phagemid Vector end: HindIII Vector end: XbaI Cloning sites: HindIII SpeI BstXI NotI XbaI Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI NsiI XbaI Construction: pUC19, RSV, bovine growth hormone Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: RSV LTR replicon: pMB1, M13 terminator: bGH polyadenylation Cross references: Vector: pRSVlacZII (phagemid) Promoters: Promoter RSV LTR Construction: pRc/RSV, pM-MuLV-SV-lacZ Marker(s):G418R,ampR,neoR Construct size (kb): 8.899999618530273 Features: marker(s): ampR, neoR, G418R promoter: RSV LTR replicon: pMB1, M13 terminator: bGH polyadenylation enhancer: RSV LTR |
| Applications： | contains sequence beta-galactosidase beta-D-galactosidase expression vector in another host, produces protein beta-galactosidase beta-D-galactosidase reporter construct shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.4, 1.2; HindIII--9.0; BglII--9.0, KpnI--4.5 (doublet); XbaI--9.0. Transient expression experiments should be performed to examine the RSV promoter efficiency in different cell lines. The RSV promoter can be replaced by excising with a BglII/HindIII digest. Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ. pRSVlacZII (TS134881), pRSVPAP (ATCC 77130) and pRSVADH (ATCC 77131) provide distinct color-staining reactions (aqua blue, red, and blue-black respectively) to permit simultaneous analysis of multiple lineages. The order of the major features in pRc/RSV is: RSV LTR - MCS - bovine growth hormone polyadenylation signal - M13 ori - SV40 early promoter - neoR - SV40 polyadenylation signal - pMB1 ori - bla. Constructed by cloning the 3.9 kb HindIII/BamHI fragment from pM-MuLV-SV-lacZ into pRc/RSV. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lin WC, Culp LA. Selectable plasmid vectors with alternative and ultrasensitive histochemical marker genes. BioTechniques 11: 344-351, 1991. PubMed: 1931036 Sanes JR, et al. Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO J. 5: 3133-3142, 1986. PubMed: 3102226 Lloyd A Culp, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |