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| 产品名称： | pMT2-V GI 5138 |
|---|---|
| 商品货号： | TS134891 |
| Designations： | pMT2-V GI 5138 |
| GenBank Number： | M16967 |
| Species： | Homo sapiens, human |
| Depositors： | Genetics Institute, Inc. |
| Applications： | in suitable host, produces protein coagulation factor V (proaccelerin, labile factor) |
| Vector： | Construct size (kb): 11.80000019073486 |
| Insert： | DNA: cDNA Insert lengths(kb): 6.908999919891357 Tissue: fetal liver Gene product: coagulation factor V (proaccelerin, labile factor) F5 Target Gene: coagulation factor V (proaccelerin, labile factor) |
| Insert Size (kb)： | 6.909 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (nt position according to published sequence): PvuII--140, 851, 2506, 2989, 5287; SacI--874, 3548; PstI--1158, 2286; EcoRI--2088, 2377, 2656, 3396, 5018; BamHI--2601, 5975, 6014; BglI--5266. The insert does not contain HindIII or SalI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 2.2, 1.9, 1.7, 0.8, 0.3; SacI--4.2, 3.8, 2.8, 0.7, 0.4; PstI--10.7, 1.2; BamHI--3.4 (doublet), 2.9, 1.9, 0.3; BglI--6.6, 4.0, 1.1, 0.2. The coding sequence extends from nt 91 to nt 6765, with the signal peptide encoded from nt 91 to nt 174. May be transformed into bacterial hosts such as E. coli HB101 or DH5, or transfected into CHO or COS cells. The vector contains dihydrofolate reductase coding sequences to permit amplification by methotrexate. Detects a mRNA of about 7.0 kb in liver and HepG2 cells. A SalI-cleavable linker was inserted into the vector EcoRI site and the insert was cloned into that SalI site. The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, and pBR322 sequences. |
| References： | Jenny RJ, et al. Complete cDNA and derived amino acid sequence of human factor V. Proc. Natl. Acad. Sci. USA 84: 4846-4850, 1987. PubMed: 3110773 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |