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| 产品名称： | Simian virus 40 |
|---|---|
| 商品货号： | TS134900 |
| Classification： | Polyomaviridae, Polyomavirus |
| Agent： | Simian virus 40 |
| Strain： | pUCSV40-B2E molecular clone of Simian virus 40, SV-40 |
| Common Name： | Simian virus 40 |
| Applications： | contains sequence |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. |
| Product Format： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of Escherichia coli containing the plasmid. |
| Storage Conditions： | Store unopened frozen vial at -80°C. |
| Comments： | This variant of SV-40-Baylor has a non-archetypal viral regulatory region and is the commonly studied version of SV-40-Baylor. Faster growing than SV-40-B1 in BSC-1, Vero, CV-1, and TC-7 cells. Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. |
| Growth Medium： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Recommended Host： | E. coli |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JA Lednicky, JS Butel |
| Special Collection： | NCRR Contract |
| References： | Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047 Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955 |