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微信小章| 产品名称： | Naegleria pringsheimi De Jonckheere |
|---|---|
| 商品货号： | TS134909 |
| Deposited As： | Naegleria gruberi Schardinger |
| Strain Designations： | CCAP 1518/1c |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | dried |
| Type Strain： | no |
| Comments： | Zymodeme Gr-4 CCAP 15181/c is no longer available from CCAP Maximum temperature tolerance 37C Equivalent to CCAP 1518/1c, Group I intron in SSUrRNA gene biochemical identification genetic cluster M Genetic cluster M RF34321 = Naegleria gruberi cluster 3 RF34247. |
| Medium： | ATCC® Medium 997: Fresh water ameba medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Cryopreservation： | 1. xa0 Allow the cells to encyst.xa0 To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Pages Balanced Salt Solution).xa0 Rub the surface of the plate with a spread bar to detach adhering amoebae. 2.xa0xa0 Transfer the cyst suspension to a sterile centrifuge tube. 3. xa0 If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration.xa0 To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106. 4.xa0xa0 While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated medium.xa0 Dissolve the DMSO by inverting the tube several times.xa0 |
| Name of Depositor： | CCAP |
| Chain of Custody： | ATCC < |
| References： | Daggett PM, Nerad TA. The biochemical identification of vahlkampfiid amoebae. J. Protozool. 30: 126-128, 1983. PubMed: 6864593 Page FC. Morphological variation in the cyst wall of Naegleria gruberi (amoebida, vahlkampfiidae). Protistologica 11: 195-204, 1975. De Jonckheere JF. A Group I intron in the SSUrDNA of some Naegleria spp. demonstrated by polymerase chain reaction amplification. J. Eukaryot. Microbiol. 40: 179-187, 1993. PubMed: 8461891 Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 Robinson BS, et al. Discontinuous genetic variation among mesophilic Naegleria isolates: Further evidence that N. gruberi is not a single species. J. Protozool. 39: 702-712, 1992. PubMed: 1453360 De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002. name change from Naegleria gruberi to N. pringsheimi per TA Nerad, by e-mail message dated Dec 9 2002 |