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微信小章| 产品名称： | pRIL6C.94 |
|---|---|
| 商品货号： | TS134928 |
| Designations： | pRIL6C.94 |
| GenBank Number： | M26744 |
| Species： | Rattus norvegicus, rat |
| Depositors： | GH Fey |
| Applications： | Linearize the plasmid with PstI and use the T7 promoter for sense-strand mRNA synthesis. A 0.9 kb BamHI-PstI fragment of the probe was used to detect a genomic BglI fragment of 13 kb in the rat and genomic BglI fragments of 23 and 6.2 kb in the mouse. |
| Vector： | Construct size (kb): 3.859999895095825 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.9010000228881836 Tissue: peritoneal exudate cell line Gene product: interleukin 6 (interferon, beta 2)(interleukin 6) IL6 |
| Insert Size (kb)： | 0.901 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.1, 1.0; PstI--3.8; BamHI--3.8. Linearize the plasmid with PstI and use the T7 promoter for sense-strand mRNA synthesis. Linearize with BamHI and use the T3 promoter for antisense-strand synthesis. IL6 has been mapped to the rat chromosome 4. This clone has the same insert as pRIL6C.91 (which was constructed in a modified Okayama and Berg vector). The insert contains nt 13 (XhoI site) through 923 (SspI site) of the full-length rat IL6 cDNA (start ATG is nt 65, stop TAG is nt 698). The mature protein is 187 amino acids, from nt 137 to nt 697. The sequence for this clone is in the GenBank/EMBL data bank with accession numbers M26744 and M26745. The derived rat amino acid sequence is 93% identical to mature murine IL6 and 58% identical to mature human IL6. A 0.9 kb BamHI-PstI fragment of the probe was used to detect a genomic BglI fragment of 13 kb in the rat and genomic BglI fragments of 23 and 6.2 kb in the mouse. |
| References： | Northemann W, et al. Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells. J. Biol. Chem. 264: 16072-16082, 1989. PubMed: 2789217 Szpirer J, et al. The Interleukin-6-dependent DNA-binding protein gene (transcription factor 5: TCF5) maps to human chromosome 20 and rat chromosome 3, the IL6 receptor locus (IL6R) to human chromosome 1 and rat chromosome 2, and the rat IL6 gene to rat chromosome 4. Genomics 10: 539-546, 1991. PubMed: 1889804 Georg H Fey, personal communication |