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微信小章| 产品名称： | pSVLfur |
|---|---|
| 商品货号： | TS135028 |
| Designations： | pSVLfur |
| GenBank Number： | X17094 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme |
| Vector： | Construct size (kb): 9.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSVL Intact vector size: 4.889 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: XhoI XbaI SmaI XmaI SacI BamHI Polylinker sites: XhoI XbaI SmaI XmaI SacI BamHI Construction: pBR322, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 late replicon: pMB1, SV40 terminator: SV40 late poly(A) Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PACE Other gene symbols: FUR Genomic copy number: unique Gene name: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) Contains complete coding sequence?: Y Chromosome: 15; Localization: 15 q26.1 Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 4.1 Cross references: DNA Seq. Acc.: X17094 Insert lengths(kb): 4.099999904632568 Gene product: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)( paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme) FUR Target Gene: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) |
| Insert Size (kb)： | 4.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 2.4; EcoRI--4.1, 2.7, 2.2; KpnI--3.9, 3.4, 1.8; PstI--3.1, 2.5, 2.0, 1.3; SmaI--5.8, 3.4. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.48, 2.42, 3.62; KpnI--0.58, 3.97; BamHI--1.84. Contains the complete coding sequence (nt 217-2601). Constructed by introducing an EcoRI site in the SmaI site of the vector. |
| References： | van den Ouweland AM, et al. Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2. Nucleic Acids Res. 18: 664, 1990. PubMed: 2408021 Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280 van de Ven WJ, et al. Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes. Mol. Biol. Rep. 14: 265-275, 1990. PubMed: 2094803 Wim J Van De Ven, personal communication |