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| 产品名称： | pAMV36 DSM PL-1006 |
|---|---|
| 商品货号： | TS135183 |
| Designations： | pAMV36 DSM PL-1006 |
| Species： | Arabis mosaic virus |
| Depositors： | EE Breyel |
| Vector： | Construct size (kb): 7.579999923706055 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.22000002861023 Gene product: genome, partial |
| Insert Size (kb)： | 3.220 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains the following sites separated by (kb): PstI - 0.45 - PstI - 0.2 - EcoRI - 0.71 - KpnI - 0.59 - HincII - 0.43 - HincII - 0.52 - HindIII - 0.32 - PstI. The 0.52 and 0.32 fragments have internal AvaI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.35; HindIII--6.6, 1.1; PstI--4.5, 2.8, 0.4. This contains two inserts of 2770 bp and 450 bp of Arabis mosaic virus RNA2. The entire virus is 4060 nt in length. Isolated by W. Jelkmann. |
| Classification： | Nepovirus |
| References： | Jelkmann W. Molekular biologische Untersuchungen zum Nachweis von Erdbeervirosen. Dissertation, Gottingen : 1987.. Jelkmann W, et al. Production and use of cDNA clones from arabis mosaic virus. Ann. Appl. Biol. 113: 483-491, 1988. Erich E Breyel, personal communication |