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| 产品名称： | Y-223a |
|---|---|
| 商品货号： | TS135209 |
| Designations： | Y-223a |
| Species： | Homo sapiens, human |
| Depositors： | U Muller, Institute of Human Genetics |
| Vector： | Construct size (kb): 5.20 Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic Insert lengths(kb): 0.75 Genome: Homo sapiens Gene symbol: DYZ5 Genomic copy number: repetitive Gene name: DNA Segment, repetitive Contains complete coding sequence?: U Chromosome: Y; Localization: Y p Tissue: 49,XXXXY cell line Type of DNA: genomic Insert end: HindIII Insert end: HindIII |
| Insert Size (kb)： | 0.75 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.2; HindIII--4.4, 0.76; NotI--uncut; EcoRI--5.2; PstI--5.2. Y-223a maps closer to the centromere than Y-280. Y-223a hybridizes to DNA in 9/16 46,XX males and a female with a dicentric Y chromosome. |
| References： | Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818 Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484 |