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| 产品名称： | pNK2809 pTN10LUK |
|---|---|
| 商品货号： | TS135230 |
| Designations： | pNK2809 pTN10LUK |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | N Kleckner |
| Vector： | Construct size (kb): 11.30000019073486 |
| Insert： | DNA: genomic Insert lengths(kb): 9.199999809265137 Gene product: ATP phosphoribosyltransferase URA3 |
| Insert Size (kb)： | 9.200 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--13.0; PstI--7.5, 5.5; EcoRI--6.4, 4.3, 2.3; BamHI--uncut; XbaI--uncut. For insertional mutagenesis of bacterial or yeast genes carried in bacteriophage lambda vectors. Derived from pNK81 by replacing 1.050 kb of uncharacterized DNA and the his promoter with 4 tandem repeats of the rrnB transcriptional terminator. Contains the mini-Tn10-LUK insert that consists of the following features: IS10R - nt 63 - promoterless lacZ element starting at codon 8 - nt 3140 - URA3 - nt 4320 - kanR - nt 6023 - IS10L - nt 6090. The order of the major features in this plasmid is: EcoRI - rrnB terminators - Tn10-LUK in hisG - hisD - EcoRI - pMB1 ori - ampR. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Huisman O, et al. A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes. Genetics 116: 191-199, 1987. PubMed: 3038670 Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |