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微信小章| 产品名称： | pVH17 |
|---|---|
| 商品货号： | TS135240 |
| Designations： | pVH17 |
| GenBank Number： | X03224 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | C Wong |
| Applications： | produces protein 2-deoxyribose-5-phosphate aldolase |
| Vector： | Construct size (kb): 5.069999217987061 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: EcoRI Vector end: AvaI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: deoC Gene name: 2-deoxyribose-5-phosphate aldolase Contains complete coding sequence?: Y Type of DNA: genomic Insert 5 end: EcoRI Insert 3 end: AvaI Insert size (kb): 2.13 Cross references: DNA Seq. Acc.: X03224 Insert lengths(kb): 2.130000114440918 Gene product: 2-deoxyribose-5-phosphate aldolase deoC Target Gene: 2-deoxyribose-5-phosphate aldolase |
| Insert Size (kb)： | 2.130 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 0.5; BglII--5.0; EcoRI/AvaI--3.0, 1.6, 0.5. The 0.5 kb EcoRI and the 1.6 kb AvaI/EcoRI fragment are from the insert. The insert contains the following restriction sites (approximate kb from the 5 end): ClaI--0.46, 0.53; EcoRI--0.48; BglII--0.59. Contains the two tandem promoter/operator regions for the deo operon, the complete coding sequence for deoC, and 53 bp of deoA. The insert includes approximately 350 bp 5 sequence not in the GenBank record. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Valentin-Hansen P, et al. The structure of tandem regulatory regions in the deo operon of Escherichia coli K12. EMBO J. 1: 317-322, 1982. Chen L, et al. Deoxyribose-5-phosphate aldolase as a catalyst in asymmetric aldol condensation. J. Am. Chem. Soc. 114: 741-748, 1992. Valentin-Hansen P, et al. The primary structure of Escherichia coli K12 2-deoxyribose 5-phosphate aldolase. Nucleotide sequence of the deoC gene and the amino acid sequence of the enzyme. Eur. J. Biochem. 125: 561-566, 1982. PubMed: 6749498 |