产品名称: | AKAP100 |
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商品货号: | TS135260 |
Designations: | AKAP100 |
GenBank Number: | U17195 |
Species: | Homo sapiens, human |
Depositors: | JD Scott |
Applications: | in suitable host, produces protein A-kinase anchor protein 100 |
Vector: | Construct size (kb): 7.639999866485596 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pET11d-kfc Intact vector size: 5.800 Type of vector: plasmid Vector end: NcoI Vector end: BamHI Cloning sites: NcoI BamHI Polylinker sites: Construction: pET11d Host range: Escherichia coli Features (with orientation and position when available): transcription terminator: T7, other: calmodulin binding domain, restriction site: KpnI restriction site: BglII other: factor X unit, restriction site: SalI other: kemptide unit, restriction site: BamHI epitope tag: T7-tag coding sequence, restriction site: NcoI promoter for in vitro transcription: T7, repressor gene: lacI, -> replicon: pMB1 marker(s): ampR, Cross references: |
Insert: | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AKAP100* Gene name: A-kinase anchor protein 100 Contains complete coding sequence?: Y Tissue: hippocampus Type of DNA: cDNA Insert 5 end: NcoI Insert 3 end: BamHI Insert size (kb): 1.965 Cross references: DNA Seq. Acc.: U17195 Nucleotides 1-1965 of the insert correspond to nucleotides 1-1965 of U17195. Insert lengths(kb): 1.9650000333786 Tissue: hippocampus Gene product: A-kinase anchor protein 100 AKAP100* Target Gene: A-kinase anchor protein 100 |
Insert Size (kb): | 1.965 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI/NcoI--5.8, 2.0; EcoRI--6.0, 1.8; HindIII--7.0, 0.6; PstI--6.2, 1.6; PvuII--3.0, 2.8, 1.5, 0.3. Constructed by ligating a PCR product creating an NcoI site on the 5 end to a fragment from a cDNA library. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.60; HindIII--1.75; NdeI--0.67; PstI--1.55; PvuII--1.31, 1.62; SacI--0.09. Transcription from the T7 promoter allows expression of a kfc-fusion protein that can be purified by affinity chromatography on calmodulin-agarose. (kfc refers to kemptide - factor X - calmodulin-binding domains). |
References: | McCartney S, et al. Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic reticulum. J. Biol. Chem. 270: 9327-9333, 1995. PubMed: 7721854 |