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| 产品名称： | pGMB |
|---|---|
| 商品货号： | TS135271 |
| Designations： | pGMB |
| GenBank Number： | X52259 |
| Species： | Homo sapiens, human |
| Depositors： | ML Lane |
| Vector： | Construct size (kb): 3.984999895095825 |
| Insert： | DNA: genomic Insert lengths(kb): 1.241999983787537 Tissue: DNA from NIH 3T3 cells transformed by MCF7 DNA Gene product: sec oncogene SEC* |
| Insert Size (kb)： | 1.242 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 1.3; EcoRI--3.9; HindIII--3.9; PstI--3.9; HincII--3.9. The insert begins 133 nucleotides 5 to nucleotide 1 of the published sequence and extends 520 nucleotides 3 to nucleotide 540 of the published sequence. The insert is oriented such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand. |
| References： | Lane MA, et al. Nucleotide sequence of a human oncogene active in tumors of secretory epithelium. Nucleic Acids Res. 18: 3068, 1990. PubMed: 2349114 Lane MA, et al. Activation of related transforming genes in mouse and human mammary carcinomas. Proc. Natl. Acad. Sci. USA 78: 5185-5189, 1981. PubMed: 6272293 Mary Ann L Lane, personal communication |