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| 产品名称： | pOC1 |
|---|---|
| 商品货号： | TS135296 |
| Designations： | pOC1 |
| Depositors： | OA Carmi |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.6000003814697300 Vector: pOC1 (plasmid) Promoters: Promoter none Marker(s):cmlR,tetR Construct size (kb): 11.60000038146973 Features: marker(s): cmlR, tetR promoter: none replicon: pMB1, pC194 |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--12.0; HindIII--8.0, 2.9; SalI--12.0; BamHI--8.5, 3.1; PstI--12.0. Light emission can be detected after promoter insertion by adding dodecyl aldehyde. A promoter cloning, Escherichia coli/Bacillus subtilis shuttle vector. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Carmi OA, et al. Use of bacterial luciferase to establish a promoter probe vehicle capable of nondestructive real-time analysis of gene expression in bacillus spp. J. Bacteriol. 169: 2165-2170, 1987. PubMed: 3106332 |
| Shipped： | freeze-dried |